year 8, Issue 1 (Spring 2014)
Iran J Med Microbiol 2014, 8(1): 54-61 |
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Shaigan nia S, Rostami F, Safarpour dehkordi F, Rahimi E, Yahaghi E, Khodaverdi Darian E et al . Isolation and Evaluation Virulence Factors of Salmonella typhimurium and Salmonella enteritidis in Milk and Dairy Products. Iran J Med Microbiol 2014; 8 (1) :54-61
URL: http://ijmm.ir/article-1-235-en.html
URL: http://ijmm.ir/article-1-235-en.html
Shima Shaigan nia 
1, Fateme Rostami2 , Farhad Safarpour dehkordi2 , Ebrahim Rahimi3 , Emad Yahaghi2 , Ebrahim Khodaverdi Darian2 , Mahrokh Bagheri Moghadam2
1, Fateme Rostami2 , Farhad Safarpour dehkordi2 , Ebrahim Rahimi3 , Emad Yahaghi2 , Ebrahim Khodaverdi Darian2 , Mahrokh Bagheri Moghadam2
1- Department of Food Science and Technology, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran , shima.shaigan@yahoo.com
2- young researchers and elite club
3- azad university of shahrekord
2- young researchers and elite club
3- azad university of shahrekord
Abstract: (23104 Views)
Background and Aim: Nutritional and economic importance of milk and dairy products is undeniable. In a day, Millions of people use from these products in their daily deals but unfortunately the hygienic quality of milk and dairy products can be changed due to presence of microbial pathogens. Salmonella enteritidis and Salmonella typhimurium are two important milk-borne pathogens. Their pathogenicity is occurred by several putative virulence genes. This study was carried out for investigate the prevalence of Salmonella spp., Salmonella enteritidis and Salmonella typhimurium in milk and dairy products and study the presence of their virulence factors.
Materials and Methods: Totally, 360 milk and dairy products were collected from Isfahan province and were immediately transferred to the Food Microbiology Research Center of Islamic Azad University of Shahrekord. All samples were cultured and their positive results were subjected to PCR method in order to determine the exact incidence rates of Salmonella spp., Salmonella enteritidis and Salmonella typhimurium and their virulence genes.
Results: Totally, 1.66% of samples were Salmonella positive. The incidence of Salmonella enteritidis and Salmonella typhimurium were 0.27% and 0.83%, respectively. Raw sheep milk had the highest prevalence of Salmonella spp. (7.5%). All of the Salmonella enteritidis and Salmonella typhimurium isolates harbored their certain virulence factors.
Conclusions: To our best knowledge the present study is the first prevalence report of Salmonella spp., Salmonella enteritidis and Salmonella typhimurium in raw sheep and goat samples in Iran. Consumption of pasteurized milk and dairy products can reduce the risk of salmonellosis.
Materials and Methods: Totally, 360 milk and dairy products were collected from Isfahan province and were immediately transferred to the Food Microbiology Research Center of Islamic Azad University of Shahrekord. All samples were cultured and their positive results were subjected to PCR method in order to determine the exact incidence rates of Salmonella spp., Salmonella enteritidis and Salmonella typhimurium and their virulence genes.
Results: Totally, 1.66% of samples were Salmonella positive. The incidence of Salmonella enteritidis and Salmonella typhimurium were 0.27% and 0.83%, respectively. Raw sheep milk had the highest prevalence of Salmonella spp. (7.5%). All of the Salmonella enteritidis and Salmonella typhimurium isolates harbored their certain virulence factors.
Conclusions: To our best knowledge the present study is the first prevalence report of Salmonella spp., Salmonella enteritidis and Salmonella typhimurium in raw sheep and goat samples in Iran. Consumption of pasteurized milk and dairy products can reduce the risk of salmonellosis.
Keywords: Salmonella typhimuriun, Salmonella enteritidis, Virulence gene, Dairy products, Isfahan province.
Type of Study: Original Research Article |
Subject:
Molecular Microbiology
Received: 2014/01/24 | Accepted: 2014/05/28 | ePublished: 2014/05/28
Received: 2014/01/24 | Accepted: 2014/05/28 | ePublished: 2014/05/28
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