Synergistic Effect of Focused Ultrasound and Albendazole Sulfoxide on Hydatid Cyst Protoscolices in Vitro

Sarmadian, Roham; Shafiee, Hadi; Bayatiani, Mohamadreza; Rokni, Mohammadbagher; Ghasemikhah, Reza

doi:10.30699/ijmm.17.6.687

year 17, Issue 6 (November - December 2023) Iran J Med Microbiol 2023, 17(6): 687-693 | Back to browse issues page

‎ 10.30699/ijmm.17.6.687

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Sarmadian R, Shafiee H, Bayatiani M, Rokni M, Ghasemikhah R. Synergistic Effect of Focused Ultrasound and Albendazole Sulfoxide on Hydatid Cyst Protoscolices in Vitro. Iran J Med Microbiol 2023; 17 (6) :687-693
URL: http://ijmm.ir/article-1-2110-en.html

Synergistic Effect of Focused Ultrasound and Albendazole Sulfoxide on Hydatid Cyst Protoscolices in Vitro

Roham Sarmadian¹

, Hadi Shafiee²

, Mohamadreza Bayatiani³

, Mohammadbagher Rokni⁴

, Reza Ghasemikhah

⁵

1- Alumna of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2- Department of Chemistry, Faculty of Science, Arak Branch, Islamic Azad University, Arak, Iran
3- Department of Radiotherapy and Medical Physics, Faculty of Paramedicine, Arak University of Medical Sciences, Arak, Iran
4- Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran , rzghasemikhah@gmail.com

Abstract: (269 Views)

Background and Aim: Hydatid cyst is caused by the larval stage of Echinococcus granulosus. This zoonotic disease causes fatalities and economic losses worldwide. Surgical and chemical treatments may be harmful or ineffective. This study evaluated ultrasonic waves and albendazole sulfoxide's synergistic effect on hydatid cyst protoscolices.
Materials and Methods: Echinococcus protoscolices from the livers of naturally infected sheep were separated into four groups. One group was exposed to 12.5 and 25 µg/mL albendazole sulfoxide for 24 hours. Another group was exposed to ultrasonic waves for 5, 9, 12, and 18 seconds at the intensities of 5, 15, and 25 w/cm², respectively. Another group was affected by ultrasound after 24 hours of Albendazole sulfoxide exposure. A control group was considered in each case. Mortality was assessed for each group and subgroup by observing protoscolices under a light microscope after adding 0.1% eosin. The data were analyzed in SPSS 23.
Results and Conclusion: Ultrasound had a greater effect on the protoscolices’ mortality than albendazole sulfoxide and the lethality was associated with intensity (F = 64.467, P=0.001) and exposure duration (F=179.932, P=0.001). Ultrasound and albendazole sulfoxide together were more effective than either alone (P=0.037), resulting in 100% death in all subgroups impacted by the combination.
Ultrasound alone can quickly destroy hydatid cyst protoscolices. Also, the synergistic effects of ultrasonic waves and albendazole sulfoxide make it possible to accomplish total protoscolices death with lower dosages of albendazole sulfoxide and shorter duration and lower intensity of ultrasonic irradiation.

Keywords: Albendazole Sulfoxide, Echinococcus Granulosus, Focused Ultrasound, Hydatid Cyst, Protoscolex, Ultrasonic Waves

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Type of Study: Brief Original Article | Subject: Medical Parasitology
Received: 2023/08/22 | Accepted: 2024/01/4 | ePublished: 2024/01/29

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