Molecular Study of Porphyromonas Gingivalis Strains With fimA Genotypes in Periodontitis Patients

F. Faruq, Zhraa; Khalaf Jabar, Sami

doi:10.30699/ijmm.17.6.663

year 17, Issue 6 (November - December 2023) Iran J Med Microbiol 2023, 17(6): 663-668 | Back to browse issues page

‎ 10.30699/ijmm.17.6.663

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F. Faruq Z, Khalaf Jabar S. Molecular Study of Porphyromonas Gingivalis Strains With fimA Genotypes in Periodontitis Patients. Iran J Med Microbiol 2023; 17 (6) :663-668
URL: http://ijmm.ir/article-1-2046-en.html

Molecular Study of Porphyromonas Gingivalis Strains With fimA Genotypes in Periodontitis Patients

Zhraa F. Faruq¹

, Sami Khalaf Jabar

1- Department of Biology, College of Science, University of Misan, Misan, Iraq
2- Department of Anatomy, College of Medicine, University of Misan, Misan, Iraq , Sami_khalaf2006@yahoo.com

Abstract: (268 Views)

Background and Aim: Porphyromonas gingivalis belongs to the phylum Bacteroidota which are non-motile, Gram-negative, rod-shaped, anaerobic, and pathogenic bacteria. It is found in the oral cavities where it is implicated in periodontal disease. This study aimed to isolate and identify Porphyromonas gingivalis from the oral cavities of patients with gingivitis and periodontal disease in Misan Governorate Center, Iraq.
Materials and Methods: The samples were collected from 50 patients aged 11-70 years old, including 21 (43%) females and 29 (57%) males. The Gram-negative pathogenic bacterial isolates were found to be the most frequent bacterial population. After incubation of bacteria on blood agar, the plates were examined for small, shiny, coccobacilli, black-pigmented, and mucoid colonies. We also tested the medical sensitivity of some pathogenic bacterial strains against antibiotics such as Cefoxitin, Doxycycline, Ciprofloxacin, and Nalidixic acid.
Results: The strains showed 100% resistance to the drugs used in the experiment. Periodontitis is caused by heterogeneous endodontic bacteria, which can be identified using species-specific PCR assays. The study successfully identified 100 anaerobic bacteria isolates using Vitek2 and 16S rDNA gene sequencing. The Porphyromonas gingivalis targeted region was found to be related to its ability.
Conclusion: Identifying and quantifying Porphyromonas gingivalis and other periodontal infections using various methods in plaque samples is crucial. Porphyromonas gingivalis has filamentous components on its cell surface called fimbriae (FimA), which are believed to be crucial for the resistance, colonization, and invasion of the periodontal tissues. The most important cause of gingivitis was found to be Porphyromonas gingivalis bacterial isolation and the age group from 31-70 or more were the most affected by gingivitis and periodontitis. According to the molecular analysis, it was also discovered that the genes of Fimbriae are among the most significant factors that promote the disease of gingivitis and periodontitis.

Keywords: Bacteria, Fimbriae, Gingivitis, Periodontitis, Polymerase Chain Reaction, Porphyromonas gingivalis

Full-Text [PDF 538 kb] (70 Downloads) | | Full-Text (HTML) (10 Views)

Type of Study: Original Research Article | Subject: Oral Microbiology
Received: 2023/10/5 | Accepted: 2024/01/10 | ePublished: 2024/01/29

References

1. Chawla N, Sarkar S. Defining "high-risk sexual behavior" in the context of substance use. J Psychosexual Health. 2019;1(1):26-31. [DOI:10.1177/2631831818822015]

2. Sanz M, Marco del Castillo A, Jepsen S, Gonzalez‐Juanatey JR, D'Aiuto F, Bouchard P, et al. Periodontitis and cardiovascular diseases: Consensus report. J Clin Periodontol. 2020;47(3):268-88. [DOI:10.1111/jcpe.13189] [PMID] [PMCID]

3. De Carvalho CC. Marine biofilms: a successful microbial strategy with economic implications. Front Mar Sci. 2018;5:126. [DOI:10.3389/fmars.2018.00126]

4. Tuck B, Watkin E, Somers A, Machuca LL. A critical review of marine biofilms on metallic materials. npj Mater Degrad. 2022;6(1):25. [DOI:10.1038/s41529-022-00234-4]

5. Holmer J. Periodontal disease and cognitive disorders. Karolinska Institutet (Sweden); 2022. Available from: [https://openarchive.ki.se/xmlui/handle/10616/47885]

6. Mohanty R, Asopa SJ, Joseph MD, Singh B, Rajguru JP, Saidath K, et al. Red complex: Polymicrobial conglomerate in oral flora: A review. J Family Med Prim Care. 2019;8(11):3480. [DOI:10.4103/jfmpc.jfmpc_759_19] [PMID] [PMCID]

7. Damgaard C, Danielsen AK, Enevold C, Massarenti L, Nielsen CH, Holmstrup P, et al. Porphyromonas gingivalis in saliva associates with chronic and aggressive periodontitis. J Oral Microbiol. 2019;11(1):1653123. [DOI:10.1080/20002297.2019.1653123] [PMID] [PMCID]

8. Ryder MI. Porphyromonas gingivalis and Alzheimer disease: Recent findings and potential therapies. J Periodontol. 2020;91:S45-9. [DOI:10.1002/JPER.20-0104] [PMID] [PMCID]

9. Endres BT, Basseres E, Citron DM, Tyrrell KL, Begum K, Lancaster C, et al. Fusobacteria behaving badly: Masquerading strains of strictly anaerobic Escherichia coli misidentified due to the deletion of the hemB gene. Anaerobe. 2023;79:102682. [DOI:10.1016/j.anaerobe.2022.102682] [PMID]

10. Chen WA, Dou Y, Fletcher HM, Boskovic DS. Local and Systemic Effects of Porphyromonas gingivalis Infection. Microorganisms. 2023;11(2):470. [DOI:10.3390/microorganisms11020470] [PMID] [PMCID]

11. Xu W, Zhou W, Wang H, Liang S. Roles of Porphyromonas gingivalis and its virulence factors in periodontitis. Adv Protein Chem Struct Biol. 2020;120:45-84. [DOI:10.1016/bs.apcsb.2019.12.001] [PMID] [PMCID]

12. Hasegawa Y, Nagano K. Porphyromonas gingivalis FimA and Mfa1 fimbriae: Current insights on localization, function, biogenesis, and genotype. Jpn Dent Sci Rev. 2021;57:190-200. [DOI:10.1016/j.jdsr.2021.09.003] [PMID] [PMCID]

13. Jia L, Han N, Du J, Guo L, Luo Z, Liu Y. Pathogenesis of important virulence factors of Porphyromonas gingivalis via toll-like receptors. Front Cell Infect Microbiol. 2019;9:262. [DOI:10.3389/fcimb.2019.00262] [PMID] [PMCID]

14. Lamont RJ, Jenkinson HF. Subgingival colonization by Porphyromonas gingivalis. Oral Microbiol Immunol: Mini‐Rev. 2000;15(6):341-9. [DOI:10.1034/j.1399-302x.2000.150601.x] [PMID]

15. Silva LM, Doyle AD, Greenwell-Wild T, Dutzan N, Tran CL, Abusleme L, et al. Fibrin is a critical regulator of neutrophil effector function at the oral mucosal barrier. Science. 2021;374(6575):eabl5450. [DOI:10.1126/science.abl5450] [PMID]

16. Chetan GB, Reddy M. Comparative evaluation of four office reconditioning methods for orthodontic stainless steel brackets on shear bond strength-an in vitro study. Ann Essences Dent. 2011;3(1):6-13. [DOI:10.5368/aedj.2011.3.1.1.2]

17. Skucaite N, Peciuliene V, Vitkauskiene A, Machiulskiene V. Susceptibility of endodontic pathogens to antibiotics in patients with symptomatic apical periodontitis. J Endod. 2010;36(10):1611-6. [DOI:10.1016/j.joen.2010.04.009] [PMID]

18. Yoshimura F, Murakami Y, Nishikawa K, Hasegawa Y, Kawaminami S. Surface components of Porphyromonas gingivalis. J Periodontal Res. 2009;44(1):1-12. [DOI:10.1111/j.1600-0765.2008.01135.x] [PMID]

19. Deppe H, Reitberger J, Behr AV, Vitanova K, Lange R, Wantia N, et al. Oral bacteria in infective endocarditis requiring surgery: a retrospective analysis of 134 patients. Clin Oral Investig. 2022;26(7):4977-85. [DOI:10.1007/s00784-022-04465-2] [PMID] [PMCID]

20. Chitsazha R, Faramarzi M, Sadighi M, Pourabbas R, Pourhajibagher M, Firouzi N, et al. Evaluation of antibacterial effect of concentrated growth factor on Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. J Family Med Prim Care. 2022;11(6):2865-9. [DOI:10.4103/jfmpc.jfmpc_2065_21] [PMID] [PMCID]

21. Parmar AS, Panwar AS. Antimicrobial Study of Chitosan-Based Crosslinked Hydrogel Against Staphylococcus aureus, Porphyromonas gingivalis, Pseudomonas aeruginosa, and Streptococcus mutans. Curr Trends Biotechnol Pharm. 2023;17(Supplement 3A):1170-83.

22. Ingalagi P, Bhat KG, Kulkarni RD, Kotrashetti VS, Kumbar V, Kugaji M. Detection and comparison of prevalence of Porphyromonas gingivalis through culture and Real Time-polymerase chain reaction in subgingival plaque samples of chronic periodontitis and healthy individuals. J Oral Maxillofac Pathol. 2022;26(2):288. [DOI:10.4103/jomfp.jomfp_163_21] [PMID] [PMCID]

23. Gan N, Qin W, Zhang C, Jiao T. One-step in situ deposition of phytic acid-metal coordination complexes for combined Porphyromonas gingivalis infection prevention and osteogenic induction. J Mater Chem B. 2022;10(22):4293-305. [DOI:10.1039/D2TB00446A] [PMID]

24. Noguchi N, Noiri Y, Narimatsu M, Ebisu S. Identification and localization of extraradicular biofilm-forming bacteria associated with refractory endodontic pathogens. Appl Environ Microbiol. 2005;71(12):8738-43. [DOI:10.1128/AEM.71.12.8738-8743.2005] [PMID] [PMCID]

25. Lan C, Chen S, Jiang S, Lei H, Cai Z, Huang X. Different expression patterns of inflammatory cytokines induced by lipopolysaccharides from Escherichia coli or Porphyromonas gingivalis in human dental pulp stem cells. BMC Oral Health. 2022;22(1):121. [DOI:10.1186/s12903-022-02161-x] [PMID] [PMCID]

26. Sugiyama N, Uehara O, Morikawa T, Paudel D, Ebata K, Hiraki D, et al. Gut flora alterations due to lipopolysaccharide derived from Porphyromonas gingivalis. Odontology. 2022;110(4):673-81. [DOI:10.1007/s10266-022-00703-x] [PMID]

27. Rolim JP. Estudos dos efeitos da terapia fotodinâmica antimicrobiana na viabilidade e na expressão gênica de S. mutans em biofilmes e lesões de cárie dentinária (Studies of the effects photodynamic therapy antimicrobial and gene expression of S. Mutans biofilms and caries dentin). Universidade Federal do Ceará. Faculdade de Farmácia, Odontologia e Enfermagem, Fortaleza, 2012. Available from: [https://repositorio.ufc.br/handle/riufc/4819]

28. Meyer HL, Abdelbary MM, Buhl EM, Kuppe C, Conrads G. Exploring the genetic and functional diversity of Porphyromonas gingivalis long fimbriae. Mol Oral Microbiol. 2023;38(5):408-23. [DOI:10.1111/omi.12433] [PMID]

29. Zou P, Li P, Liu J, Cao P, Luan Q. Direct current exerts electricidal and bioelectric effects on Porphyromonas gingivalis biofilms partially via promoting oxidative stress and antibiotic transport. J Microbiol. 2022;60(1):70-8. [DOI:10.1007/s12275-022-1238-5] [PMID]

30. Moreno S, Jaramillo A, Parra B, Botero JE, Contreras A. Porphyromonas gingivalis Fim-A genotype distribution among Colombians. Colombia Médica. 2015;46(3):122-7. [DOI:10.25100/cm.v46i3.1535] [PMID] [PMCID]