Detection of Biologically Active Compounds in Eriobotrya japonica L. Seeds Extract and Determination of Their Effectiveness Against Dermatophytes

Basheer Bahedh, Shahad; Yousif Mohammed, Dina

doi:10.30699/ijmm.17.6.680

year 17, Issue 6 (November - December 2023) Iran J Med Microbiol 2023, 17(6): 680-686 | Back to browse issues page

‎ 10.30699/ijmm.17.6.680

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Basheer Bahedh S, Yousif Mohammed D. Detection of Biologically Active Compounds in Eriobotrya japonica L. Seeds Extract and Determination of Their Effectiveness Against Dermatophytes. Iran J Med Microbiol 2023; 17 (6) :680-686
URL: http://ijmm.ir/article-1-1769-en.html

Detection of Biologically Active Compounds in Eriobotrya japonica L. Seeds Extract and Determination of Their Effectiveness Against Dermatophytes

Shahad Basheer Bahedh

¹, Dina Yousif Mohammed²

1- Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq , shahadbasheer92@gmail.com
2- Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq

Abstract: (296 Views)

Background and Aim: Effective dermatophytosis treatment necessitates antibiotics, which can harm the body's health. This systematic review study aims to evaluate the susceptibility profile of dermatophytes to plant extracts, and to address this critical gap in understanding resistance and treatment of dermatophytes.
Materials and Methods: Eriobotrya japonica seeds were collected, ground, and extracted, and then GC-MS analysis was performed to detect secondary compounds. Then, treatments of the plant extract (Ethanol) were made at a concentration (300, 150 and 75 mg/mL) with a control treatment (-) and a control treatment (+) containing a medicinal antifungal (Nystatin), treating it against dermatophytes and indicating the rate of inhibition for each isolate of dermatophytes (Trichophyton rubrum, Trichophyton mentographytes, Microsporum canis, Trichophyton verrucosum, Trichophyton quinckeanum, Epidermophyton, Trichophyton simii, Trichophyton interdigitale, and Fusarium).
Results: The seed extract significantly inhibited dermatophyte growth at 300 mg/mL concentration, with percentage inhibition growth ranging from 100% to 96.56%, compared to concentrations at 150 and 75 mg/mL, with percentage inhibition growth ranging from 97.27% to 86.66% and from 93.66% to 79.84%, respectively. The results of GC-MS analysis indicated that the ethanol seeds extract of loquat has a lot of active chemical compounds, including twenty-five phytoconstituent compounds that are distinguished in the extract of seeds with E. japonica L. In addition, the high area percentages revealed in each extract were Propylene glycol monooleate 5.03%, D-Mannitol or DL-Glucitol 5.67%, Beta-sitosterol 5.70%, n-hexadecanoic acid 14.53% and 9,12-Octadecadienoic acid (Z, Z)- 22.85%.
Conclusion: Based on our results, the ethanolic extract of the loquat plant was effective in inhibiting the growth of most dermatophytes to a significant extent, but the Fusarium genus was less sensitive. This is useful in choosing plant extracts in treating dermatophytes, which confirms their importance and their containment of beneficial active compounds.

Keywords: Antifungal, Dermatophytes, Eriobotrya japonica, Ethanol Extract, Seeds

Full-Text [PDF 538 kb] (52 Downloads) | | Full-Text (HTML) (16 Views)

Type of Study: Original Research Article | Subject: Medical Mycology
Received: 2023/09/19 | Accepted: 2024/01/15 | ePublished: 2029/01/29

References

1. AL-Khikani FH. Dermatophytosis a worldwide contiguous fungal infection: Growing challenge and few solutions. Biomed Biotechnol Res J. 2020;4(2):117-22. [DOI:10.4103/bbrj.bbrj_1_20]

2. Al-Janabi AA. Dermatophytosis: Causes, clinical features, signs and treatment. J Symptoms Signs. 2014;3(3):200-3.

3. Mei A, Ricciardo B, Raby E, Kumarasinghe SP. Plant-based therapies for dermatophyte infections. Tasman Med J. 2022;4(3):21-37.

4. Al-Ghazali NA, Hameed ZL, Abu-Duka AB. First Report of Aspergillus Leaf Spot on Loquat (Eriobotrya japonica) Caused by Aspergillus fumigatus in Iraq. In IOP Conference Series: Earth and Environmental Science. IOP Publishing. 2023;1158(4):042030. [DOI:10.1088/1755-1315/1158/4/042030]

5. Jian T, Chen J, Ding X, Lv H, Li J, Wu Y, et al. Flavonoids isolated from loquat (Eriobotrya japonica) leaves inhibit oxidative stress and inflammation induced by cigarette smoke in COPD mice: the role of TRPV1 signaling pathways. Food Funct. 2020;11(4):3516-26. [DOI:10.1039/C9FO02921D] [PMID]

6. Henmi A, Shoji M, Nomura M, Inoue T. Fatty acid composition and applications of Eriobotrya japonica seed oil. J Oleo Sci. 2019;68(7):599-606. [DOI:10.5650/jos.ess18178] [PMID]

7. Inoue M, Hayashi S, Craker L. Culture, history, and applications of medicinal and aromatic plants in Japan. In Aromatic and Medicinal Plants-Back to Nature. Intechopen Publishing. 2017:95-110. [DOI:10.5772/66505]

8. Cornejal N, Pollack E, Kaur R, Persaud A, Plagianos M, Juliani HR, et al. Antimicrobial and Antioxidant Properties of Theobroma cacao, Bourreria huanita, Eriobotrya japonica, and Elettaria cardamomum-Traditional Plants Used in Central America. J Med Act Plants. 2023;12(1):1-17.

9. Liu Y, Zhang W, Xu C, Li X. Biological activities of extracts from loquat (Eriobotrya japonica Lindl.): a review. Int J Mol Sci. 2016;17(12):1983. [DOI:10.3390/ijms17121983] [PMID] [PMCID]

10. Rashed KN, Butnariu M. Isolation and antimicrobial and antioxidant evaluation of bio-active compounds from Eriobotrya japonica stems. Adv Pharm Bull. 2014;4(1):75-81.

11. Abubakar AR, Haque M. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. J Pharm Bioallied Sci. 2020;12(1):1-10. [DOI:10.4103/jpbs.JPBS_175_19] [PMID] [PMCID]

12. Eswaran R, Anandan A, Doss A, Sangeetha G, Anand SP. Analysis of chemical composition of Cissus quadrangularis linn by GC-MS. Asian J Pharm Clin Res. 2012;5(Supple 2):139-40.

13. Wang SY, Wu CL, Chu FH, Chien SC, Kuo YH, Shyur LF, et al. Chemical composition and antifungal activity of essential oil isolated from Chamaecyparis formosensis Matsum. wood. Holzforschung. 2005;59(3):295-9. [DOI:10.1515/HF.2005.049]

14. Abdu B. Comparative study of antimicrobial potentials of seed oils of jatropha curcas and Tamarindus indica. Int J Sci Res Sci Eng Technol. 2019;6(5):29-36.

15. Mehdi MA, Alawi AH, Thabet AZ, Alarabi F, Omar GM, Pradhan V. Analysis of bioactive chemical compounds of leaves extracts from Tamarindus indica using FT-IR and GC-MS spectroscopy. Asian J Res Biochem. 2021;8(1):22-34. [DOI:10.9734/ajrb/2021/v8i130171]

16. Fagbemi KO, Aina DA, Olajuyigbe OO. Soxhlet extraction versus hydrodistillation using the clevenger apparatus: A comparative study on the extraction of a volatile compound from Tamarindus indica seeds. Sci World J. 2021;2021:1-8. [DOI:10.1155/2021/5961586] [PMID] [PMCID]

17. Khidir A, Abdel Karim M. Constituents and Antimicrobial Activity of Oil from Withania somnifera Grown in Sudan. J Faculty Sci Technol. 2022;9 (2):117-24.

18. Cui K, He L, Cui G, Zhang T, Chen Y, Zhang T, et al. Biological activity of trans-2-hexenal against the storage insect pest Tribolium castaneum (Coleoptera: Tenebrionidae) and mycotoxigenic storage fungi. J Econ Entomol. 2021;114(2):979-87. [DOI:10.1093/jee/toab001] [PMID]

19. Neto LJ, Ramos AG, Freitas TS, Barbosa CR, de Sousa Júnior DL, Siyadatpanah A, et al. Evaluation of benzaldehyde as an antibiotic modulator and its toxic effect against Drosophila melanogaster. Molecules. 2021;26(18):5570. [DOI:10.3390/molecules26185570] [PMID] [PMCID]

20. Ogala JB, Hassan Y, Samaila A, Bindawa MI, TOK TT. Synthesis, antifungal activity and in silico ADMET studies of benzyl alcohol derivatives. İstanbul J Pharm. 2022;52(1):47-53. [DOI:10.26650/IstanbulJPharm.2022.958484]

21. Mahdavi SM, Habibi A, Dolati H, Shahcheragh SM, Sardari S, Azerang P. Synthesis and antimicrobial evaluation of 4H-pyrans and schiff bases fused 4H-pyran derivatives as inhibitors of Mycobacterium bovis (BCG). Iran J Pharm Res. 2018;17(4):1229-39.

22. Joye, IJ. Acids and Bases in Food. Editor(s): Laurence Melton, Fereidoon Shahidi, Peter Varelis, Encyclopedia of Food Chemistry. Academic Press. 2019:1-9. [DOI:10.1016/B978-0-08-100596-5.21582-5] [PMID]

23. Abdou A, Elmakssoudi A, El Amrani A, JamalEddine J, Dakir M. Recent advances in chemical reactivity and biological activities of eugenol derivatives. Med Chem Res. 2021;30:1011-30. [DOI:10.1007/s00044-021-02712-x]

24. Kim J, Kim YY, Chang JY, Kho HS. Candidacidal activity of xylitol and sorbitol. J Oral Med Pain. 2016;41(4):155-60. https://doi.org/10.14476/jomp.2016.41.4.155 [DOI:10.14476/jomp.2021.46.4.155]

25. Shobi T, Viswanathan M. Antibacterial activity of di-butyl phthalate isolated from Begonia malabarica. J Appl Biol Biotechnol. 2018;5(2):97-100. [DOI:10.15406/jabb.2018.05.00123]

26. Lima R, Fernandes C, Pinto MM. Molecular modifications, biological activities, and applications of chitosan and derivatives: A recent update. Chirality. 2022;34(9):1166-90. [DOI:10.1002/chir.23477] [PMID]

27. Huang L, Zhu X, Zhou S, Cheng Z, Shi K, Zhang C, et al. Phthalic acid esters: Natural sources and biological activities. Toxins. 2021;13(7):495. [DOI:10.3390/toxins13070495] [PMID] [PMCID]

28. Sbihi HM, Nehdi IA, Mokbli S, Romdhani-Younes M, Al-Resayes SI. Hexane and ethanol extracted seed oils and leaf essential compositions from two castor plant (Ricinus communis L.) varieties. Ind Crops Prod. 2018;122:174-81. [DOI:10.1016/j.indcrop.2018.05.072]

29. Castejón N, Luna P, Señoráns FJ. Alternative oil extraction methods from Echium plantagineum L. seeds using advanced techniques and green solvents. Food Chem. 2018;244:75-82. [DOI:10.1016/j.foodchem.2017.10.014] [PMID]

30. Adham D, Taufiqurrahman I, Helmi ZN. Flavonoid level analysis of Binjai leaf extract (Mangifera caesia) in ethanol, methanol, and n-hexane solvents. 2019;4(1):46-9.

31. Scheibler E, Garcia MC, Medina da Silva R, Figueiredo MA, Salum FG, Cherubini K. Use of nystatin and chlorhexidine in oral medicine: Properties, indications and pitfalls with focus on geriatric patients. Gerodontology. 2017;34(3):291-8. [DOI:10.1111/ger.12278] [PMID]

32. Muraih JK, Arean AG, Abdulabass HT. Phytochemical and antibacterial activity of Capparis spinosa roots extracts against some pathogenic bacteria. Ann Trop Med Public Health. 2020;23(S10):SP231010. [DOI:10.36295/ASRO.2020.231010]